Journal of Laboratory Physicians
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Year : 2009  |  Volume : 1  |  Issue : 2  |  Page : 69-72

Optimum time to detection of bacteria and yeast species with BACTEC 9120 culture system from blood and sterile body fluids

Department of Microbiology, SMS Medical College, Jaipur, India

Correspondence Address:
Nita Pal
Department of Microbiology, SMS Medical College, Jaipur
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Source of Support: None, Conflict of Interest: None

DOI: 10.4103/0974-2727.59703

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The culture results of 4,807 blood and 383 sterile body fluid specimens received in our laboratory during a 54-month period, were analyzed to determine the time required for culture to become positive, time at which a culture could safely be considered negative, and the spectrum of isolated organisms. The specimens were processed by automated BACTEC 9120 culture system. A total of 1,677 clinically significant microorganisms were isolated. Gram positive and negative bacterial isolation rates were found to be 62.55% and 32.20%, respectively. Yeasts were recovered in 5.24%. False positivity rate was 1.5%. Clinically significant isolates recovered on day four and five were 97.81% and 99.88%, respectively. At day five, the sensitivity was 99.94% and negative predictive value 99.96%. Our data support a five-day incubation protocol for recovery of all clinically significant organisms with sensitivity reduced by 0.06%, when compared with a six-day protocol.

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