Journal of Laboratory Physicians
Home About us Ahead of print Current issue Back issues Subscribe Instructions Contact Login 
Wide layoutNarrow layoutPrint this page  Email this page Bookmark this page Small font size Default font size Increase font size 
 
ORIGINAL ARTICLE
Year : 2010  |  Volume : 2  |  Issue : 2  |  Page : 93-99

Detection of viral citrullinated peptide antibodies directed against EBV or VCP: In early rheumatoid arthritis patients of Indian origin


1 Sir. H.N. Medical Research Society; Sir. H.N. Hospital and Research Centre, Raja Ram Mohan Roy Road, Girgaum, Mumbai - 400 004, India
2 Sir. H.N. Hospital and Research Centre, Raja Ram Mohan Roy Road, Girgaum, Mumbai - 400 004, India

Correspondence Address:
Sudha S Deo
Sir. H.N. Medical Research Society; Sir. H.N. Hospital and Research Centre, Raja Ram Mohan Roy Road, Girgaum, Mumbai - 400 004
India
Login to access the Email id

Source of Support: Mentioned in acknowledgement, Conflict of Interest: None


DOI: 10.4103/0974-2727.72158

Rights and Permissions

Aim : Study was undertaken to analyze the frequency of anti-viral citrullinated peptide (anti-VCP) antibodies in sera from patients with early rheumatoid arthritis (ERA). Materials and Methods : Viral citrullinated peptide (VCP) and Epstein-Barr nuclear antigen (EBNA-1) peptide were commercially prepared and antibodies to these were determined in 25 patients of ERA, 40 disease control patients constituting 25 rheumatoid arthritis (RA), 7 systemic lupus erythematosus (SLE), 2 scleroderma, 1 spondyloarthritis (SpA), 1 juvenile rheumatoid arthritis (JRA), 1 osteoarthritis (OA), 1 psoriatic arthritis (PsA), 1 undifferentiated arthritis (UA), and 1 gout and 25 healthy controls (HCs) were taken for comparison. In-house ELISA was established for both the antibodies while cyclic citrullinated peptide (CCP) antibody was detected by commercial ELISA kit. Results : Significant increase in VCP antibody by ERA and disease controls than healthy normal was observed. VCP IgM antibody was significantly increased in RA patients than HC. The presence of VCP antibody signifies a good marker for ERA. We observed significant difference in the VCP IgG and IgM antibody when compared to EBNA-1. In-house ELISA established for EBNA-1 and VCP antibodies showed low sensitivity but 96% specificity. Conclusions : We observed that sera from early RA patients reacted to the deiminated protein encoded by Epstain Barr Virus (EBV). Thus a possible role of virus in inducing an anti-citrullinated peptide antibody (ACPA) response reveals viral etiology in this disease.


[FULL TEXT] [PDF]*
Print this article     Email this article
 Next article
 Previous article
 Table of Contents

 Similar in PUBMED
   Search Pubmed for
   Search in Google Scholar for
 Related articles
 Citation Manager
 Access Statistics
 Reader Comments
 Email Alert *
 Add to My List *
 * Requires registration (Free)
 

 Article Access Statistics
    Viewed2715    
    Printed195    
    Emailed2    
    PDF Downloaded147    
    Comments [Add]    

Recommend this journal