Journal of Laboratory Physicians
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ORIGINAL ARTICLE
Year : 2012  |  Volume : 4  |  Issue : 1  |  Page : 30-34

Rapid identification of yeast isolates from clinical specimens in critically Ill trauma ICU patients


1 Department of Microbiology (Laboratory Medicine), Jai Prakash Narayan Apex Trauma Centre, New Delhi, India
2 Department of Laboratory Medicine, Jai Prakash Narayan Apex Trauma Centre, New Delhi, India
3 Department of Surgical Disciplines, All India Institute of Medical Sciences, New Delhi, India
4 Department of Microbiology, All India Institute of Medical Sciences, New Delhi, India

Correspondence Address:
Purva Mathur
Department of Laboratory Medicine, Jai Prakash Narayan Apex Trauma Centre, New Delhi
India
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/0974-2727.98667

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Purpose: The purpose was to evaluate the performance of a commercially available chromogenic Candida speciation media and the Vitek 2 ID system for the identification of medically important yeasts and yeast-like organisms in a routine clinical microbiology laboratory. Materials and Methods: A total of 429 non duplicate, consecutive yeast strains were included during the 3.5-year study period. The performance of the Vitek 2 ID system and a chromogenic agar medium was evaluated against the gold standard conventional phenotypic and biochemical identification method for speciation of yeast isolates from trauma patients. Results: Candida tropicalis (64%) was the most common Candida species, followed by Candida albicans (14%), Candida rugosa (7%), and Candida parapsilosis (6.5%). Of the 429 isolates, 183 could be identified to species level by all the three methods. Agreement between the chromogenic agar method and conventional methods was 80% for Candida tropicalis, 100% for Candida rugosa, 89% for Candida albicans, and 77% for Candida parapsilosis. Vitek 2 had lower sensitivity, with agreement of 49% for Candida tropicalis, 100% for Candida rugosa, 39% for Candida albicans, and 31% for Candida parapsilosis. Conclusion: Thus, in long-term ICU patients, an increasing trend of isolating nonalbicans Candida spp. continues. The chromogenic agar medium is a convenient and economic method to identify commonly isolated species in busy clinical microbiology laboratories.


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