Journal of Laboratory Physicians
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HOW I DO IT
Year : 2012  |  Volume : 4  |  Issue : 2  |  Page : 112-119

Rapid Identification and Drug Susceptibility Testing of Mycobacterium tuberculosis: Standard Operating Procedure for Non-Commercial Assays: Part 2: Nitrate Reductase Assay v1.3.12


1 Department of Laboratory Medicine, Division of Clinical Microbiology, All India Institute of Medical Sciences, New Delhi, India; Culture and Drug Susceptibility Testing Subgroup, New Diagnostic Working Group, STOP TB Partnership, Geneva, Switzerland
2 Department of Laboratory Medicine, Division of Clinical Microbiology, All India Institute of Medical Sciences, New Delhi, India
3 Department of Medical Microbiology, School of Biomedical Sciences, Makerere University College of Health Sciences, Kampala, Uganda; Culture and Drug Susceptibility Testing Subgroup, New Diagnostic Working Group, STOP TB Partnership, Geneva, Switzerland
4 Department of Biochemistry and Microbiology, Ghent University, Belgium; Culture and Drug Susceptibility Testing Subgroup, New Diagnostic Working Group, STOP TB Partnership, Geneva, Switzerland
5 Culture and Drug Susceptibility Testing Subgroup, New Diagnostic Working Group, STOP TB Partnership, Geneva, Switzerland

Correspondence Address:
Sarman Singh
Department of Laboratory Medicine, Division of Clinical Microbiology, All India Institute of Medical Sciences, New Delhi, India; Culture and Drug Susceptibility Testing Subgroup, New Diagnostic Working Group, STOP TB Partnership, Geneva, Switzerland

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Source of Support: Culture and drug susceptibility testing subgroup, Stop-TB Partnership, WHO & FIND, Geneva, Switzerland., Conflict of Interest: None


DOI: 10.4103/0974-2727.105593

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In the previous part, we presented the standard operating procedure (SOP) of the microscopic observation drug susceptibility assay drug susceptibility testing (DST) for Mycobacterium tuberculosis. The present SOP is devoted to another non-commercial culture and DST method known as nitrate reductase assay (NRA). As the name implies, the NRA detects the ability of M. tuberculosis to reduce nitrate to nitrite. In the assay, the presence of nitrite is detected by the addition of p-nitrobenzoate into the growth yield. The reaction is detected by the naked eye. The incorporation of drugs in the medium allows to use the test for DST, which can be interpreted with naked eyes. The identification and drug susceptibility results can be obtained in 2-3 weeks. This SOP document has been developed through the culture and DST subgroup of the STOP tuberculosis (TB) Partnership New Diagnostic Working Group. It is intended for laboratories that would want to use or already using this rapid non-commercial method for culture identification and DST of M. tuberculosis, notably in resource-constraint settings in Asia and Africa.


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