Journal of Laboratory Physicians
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   Table of Contents     
CASE REPORT
Year : 2013  |  Volume : 5  |  Issue : 2  |  Page : 133-135  

Urinary tract infection due to Paenibacillus alvei in a chronic kidney disease: A rare case report


Department of Microbiology, Maharaja Krishna Chandra Gajapati Medical College and Hospital, Berhampur, Odisha, India

Date of Web Publication15-Oct-2013

Correspondence Address:
Sanghamitra Padhi
Department of Microbiology, Maharaja Krishna Chandra Gajapati Medical College and Hospital, Berhampur, Odisha
India
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/0974-2727.119872

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   Abstract 

Paenibacilli, the Gram positive, aerobic spore bearing bacilli are found normally in the environment. Though these organisms were not known to cause human disease, until recently; few species of this genus have been reported to cause infections in humans. We report here, a case of urinary tract infection in a 60-year-old chronic kidney disease patient due to this rare bacterium. The patient presented with complains of fever, dysuria, and flank pain. Routine and microscopic examination of urine revealed no abnormality except plenty of pus cells and albumin (1+). Bacterial culture showed significant bacteruria and the isolated bacteria was identified to be Paenibacillus alvei based on standard biochemical reactions.

Keywords: Chronic kidney disease, Paenibacillus alvei, urinary tract infection


How to cite this article:
Padhi S, Dash M, Sahu R, Panda P. Urinary tract infection due to Paenibacillus alvei in a chronic kidney disease: A rare case report. J Lab Physicians 2013;5:133-5

How to cite this URL:
Padhi S, Dash M, Sahu R, Panda P. Urinary tract infection due to Paenibacillus alvei in a chronic kidney disease: A rare case report. J Lab Physicians [serial online] 2013 [cited 2019 Dec 7];5:133-5. Available from: http://www.jlponline.org/text.asp?2013/5/2/133/119872


   Introduction Top


Paenibacillus is a genus of aerobic, Gram-positive bacilli, originally included in the genus Bacillus has been separated from it based on phylogenetic studies using 16S rRNA gene. [1] Several species of Paenibacillus have been reported to cause human infections like Paenibacillus alvei in prosthetic hip infections, cellulitis, and endophthalmitis; [2],[3],[4] P. macerans in brain abscess, catheter associated infection, and wound infection; [5],[6] P. polymyxa in bacteremia. [7]

We now report a case of urinary tract infection in a chronic kidney disease patient caused by Paenibacillus alvei, which to the best of our knowledge is first to be reported.


   Case Report Top


The patient is a 60-year-old male, farmer by occupation, residing in a village complained of dysuria and fever for which he consulted the local physician. He was advised to do the minimum available investigations, which showed fasting blood sugar 81 mg/dL; hemoglobin (Hb) 9.6 gm/dL; and total leukocyte count 11,200 cells/mm 3 . His differential count revealed increased percentage of neutrophil (85%). Routine and microscopic examination of urine showed presence of albumin (1+), plenty of pus cells, and 2-3 epithelial cells per high power field. Based on this report, the patient was advised oral administration of Norfloxacin 400 mg twice daily for 7 days. But 12 days later, patient complained of right side flank pain and increased intensity of fever for last 8 days. So he was referred to the tertiary care hospital for consultation, where different investigations were carried out. Ultrasonography of abdomen revealed hypoplastic right kidney [Figure 1]. Serum urea and creatinine levels were 56 and 1.3 mg/dL, respectively. The patient was diagnosed as a case of chronic kidney disease and his urine sample was sent to the Department of Microbiology for different investigations. Routine and microscopic examination of urine revealed presence of albumin (1+), plenty of pus cells, 1-2 epithelial cells per high power field. Bacteriological culture of urine showed significant bacteriuria (10 5 cfu/ml) and the colonies [Figure 2] were moist, glossy with an unpleasant odor. Gram staining of colonies detected presence of Gram-positive bacilli bearing ellipsoidal, subterminal spores [Figure 3]. By standard biochemical reactions, the isolate was identified as Paenibacillus alvei. [8] A repeat culture of the urine sample was performed to confirm its isolation. The isolate was sensitive to ampicillin, amikacin, nitrofurantoin, cefotaxime, cefixime, and resistant to norfloxacin. Hence, the patient was administered with intravenous injection of 1 g of cefotaxime 8-hourly for 7 days. Then a repeat urine culture was performed which was found to be bacteriologically sterile.
Figure 1: Ultrasonography showing hypoplastic kidney

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Figure 2: Growth and isolation of bacteria on the culture plate

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Figure 3: Gram staining showing subterminal spore in Gram-positive bacilli

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   Discussion Top


Paenibacillus, a genus of Gram positive, facultative an aerobic, endospore forming bacteria, belongs to the family Paenibacillaceae. The name paeni has been derived from the latin word paene which means almost and so the Paenibacilli are literally almost Bacilli. Because of highly conserved genome encoding their 16S rRNA which differs from that of Bacillus, they were included in a novel genus called Paenibacillus which consists of more than 90 species. Many species of this genus have already been isolated from clinical samples among which Paenibacillus alvei is the commonest one. P. alvei has also been isolated from life threatening conditions like meningitis and pneumonitis. [9],[10] P. alvei originally isolated from honeycomb of bees and later on from soil, is found to produce thiol-activated cytolysins which might be responsible for its virulence.

In our case, elevated neutrophil count and plenty of pus cells in microscopic examination of urine, on two occasions, in a febrile patient was indicative of urinary tract infection. In addition to this isolation of P. alvei from urine culture, from a chronic kidney disease patient, and bacteriological sterile urine after 7 days of antimicrobial administration is sufficient enough to say that the urinary tract infection was due to P. alvei.

Cultures showing isolation of Gram-positive spore bearing bacilli are in general considered as contaminants as they are ubiquitous in nature. But isolation of these bacilli in pure form, that to in any preexisting disease condition and immunocompromised conditions should not be neglected and be processed for identification to reduce the morbidity and mortality of the patients.

 
   References Top

1.Ash C, Priest FG, Collins MD. Molecular identification of rRNA group 3 bacilli (Ash, Farrow, Wallbanks and Collins) using a PCR probe test. Proposal for the creation of a new genus Paenibacillus. Antonie Van Leeuwenhoek 1993;64:253-60.  Back to cited text no. 1
    
2.Reboli AC, Bryan CS, Farrar WE. Bacteremia and infection of a hip prosthesis caused by Bacillus alvei. J Clin Microbiol 1989;27:1395-6.  Back to cited text no. 2
    
3.Shin JH, Sung SK, Shim JD, Kim HR, Park SW, Lee JN. A case of Paenibacillus alvei cellulitis in immune-competent patient. Korean J Lab Med 2005;25:53-5.  Back to cited text no. 3
    
4.Antonello A, Weinstein GW. Successful treatment of Bacillus alvei endophthalmitis. Am J Ophthalmol 1989;108:454-5.  Back to cited text no. 4
    
5.Bert F, Ouahes O, Lambert-Zechovsky N. Brain abscess due to Bacillus macerans following a penetrating periorbital injury. J Clin Microbiol 1995;33:1950-3.  Back to cited text no. 5
    
6.Barrero F, Galán F, Marín P, García-Martos P, Capote FJ. Catheter associated infection by Bacillus macerans in a patient with acute leukemia. Enferm Infecc Microbiol Clin 1996;14:628-9.  Back to cited text no. 6
    
7.Nasu Y, Nosaka Y, Otsuka Y, Tsuruga T, Nakajima M, Watanabe Y, et al. A case of Paenibacillus polymyxa bacteremia in a patient with cerebral infarction. Kansenshogaku Zasshi 2003;77:844-8.  Back to cited text no. 7
    
8.Logan NA. Bacillus anthracis, Bacillus cereus, and other aerobic endospore-forming bacteria, In: Borriello SP, Murray PR, Funke G, editors. Topley and Wilson's Microbiology and Microbial Infections. 10 th ed. ASM Press, Hodder Arnold; 2005. p. 922-52.  Back to cited text no. 8
    
9.Park SJ, Cong W, Lee SY. Bacterial and fungal species from cerebrospinal fluid in the past five years. Korean J Pathol 1976;10:137-42.  Back to cited text no. 9
    
10.Coudron PE, Payne JM, Markowitz SM. Pneumonia and empyema infection associated with a Bacillus species that resembles B. alvei. J Clin Microbiol 1991;29:1777-9.  Back to cited text no. 10
    


    Figures

  [Figure 1], [Figure 2], [Figure 3]



 

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