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In silico analysis of tcr vβ7 of two patients with type 1 diabetes mellitus
Jianwei Zhou1, Cui Kong2, Xiukui Wang3, Yinfeng Jia1, Li Wang4, Hong Chang1, Lin Sun5
1 Clinic Laboratory, The Affiliated Hospital of Jining Medical College, Jining, Shandong Province, China
2 Department of Cardiovascular Disease, The Affiliated Hospital of Jining Medical College, Jining, Shandong Province, China
3 Department of Stomatology, The Affiliated Hospital of Jining Medical College, Jining, Shandong Province, China
4 Department of Pharmocology, The First People's Hospital, Jining, Shandong Province, China
5 Department of Endocrinology, The Affiliated Hospital of Jining Medical College, Jining, Shandong Province, China
Correspondence Address:
Jianwei Zhou
Clinic Laboratory, The Affiliated Hospital of Jining Medical College, Jining, Shandong Province
China
 Source of Support: None, Conflict of Interest: None  | Check |
DOI: 10.4103/0974-2727.119845
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Objective: To compare the sequences and crystal structures of variable region of beta chain 7 (Vβ7) of T cell receptor (TCR) of two patients with type 1 diabetes mellitus (T1DM).
Patients and Methods: The skewness of TCR Vβ7 of two T1DM patients were detected with real-time florescence quantitative polymerase chain reaction (FQ-PCR) and deoxyribonucleic acid (DNA) melting curve analysis technique followed by being sequenced, the crystal structures of them were simulated according to CPH models 2.0 Server, IMGT database, and RasMol 2 software.
Results: The whole sequences of TCR Vβ7 of T1DM patient-1 were "CASRTAGQYEQYFGPGTR", that of patient-2 were "CASRTAGQYEQFFGPGTR"; the only difference between them lied on the 12 th amino acid. The crystal structures of Vβ7 of the two patients simulated with backbone model were rather similar, while that with sphere model were obviously different.
Conclusion: Although the TCR Vβ7 of the T1DM patients share the similar gene sequences, their crystal structures simulated with sphere model are different, and the mechanism needs further study. |
