|Year : 2018 | Volume
| Issue : 3 | Page : 299-303
Direct identification and susceptibility testing of Gram-negative bacilli from turbid urine samples using VITEK2
Neelima Angaali1, Laxmi Vemu2, Chavali Padmasri1, Neeraja Mamidi1, Vijay Dharma Teja1
1 Department of Microbiology, NIMS, Hyderabad, Telangana, India
2 Department of Microbiology, Kamineni Life Sciences, Hyderabad, Telangana, India
|Date of Submission||24-Jun-2017|
|Date of Acceptance||13-Nov-2017|
|Date of Web Publication||06-Jul-2018|
Dr. Neelima Angaali
H.NO. 3-6-536, Raja Residency, Flat- 201, Street No. 7, Himayat Nagar, Hyderabad - 500 029, Telangana
Source of Support: None, Conflict of Interest: None
| Abstract|| |
INTRODUCTION: Urinary tract infections (UTIs) are the most common infectious diseases occurring in either the community or healthcare setting. Turnaround time for urine culture is about 24 h, and antimicrobial susceptibility testing (AST) requires another 24 h. Consequently, initial antibiotic therapy is mostly empirical.
MATERIALS AND METHODS: This study was conducted at Nizam's Institute of Medical Sciences, Hyderabad. Turbid urine samples which showed pus cells and Gram-negative (GN) bacilli of single morphotype were included. The turbidity of the urine was adjusted to 0.5 McFarland and uploaded directly in the VITEK 2 identification (ID) GN and N-280 panel for AST. The specimen was also inoculated on CHROMagar, and the ID and AST of the isolates from the agar plate were repeated on VITEK 2, and the results were compared.
RESULTS: Out of 844 turbid urines screened, 62 met the inclusion criteria. Escherichia coli was the most common isolate (71.9%). Complete agreement for ID was 80.7%, misidentified were 12.2%, and unidentified were 7%. Complete agreement with AST was 94.3%, very major errors 0.5%, major errors 2.2%, and minor errors 3%.
CONCLUSION: With a 94.3% agreement for AST and a reduced turnaround time by 24 h, the direct inoculation had a potential clinical benefit for initiating timely and appropriate antibiotic therapy for UTI.
Keywords: Complete agreement, major errors, minor errors, very major errors, VITEK 2 IDGN, VITEK 2 N280
|How to cite this article:|
Angaali N, Vemu L, Padmasri C, Mamidi N, Teja VD. Direct identification and susceptibility testing of Gram-negative bacilli from turbid urine samples using VITEK2. J Lab Physicians 2018;10:299-303
|How to cite this URL:|
Angaali N, Vemu L, Padmasri C, Mamidi N, Teja VD. Direct identification and susceptibility testing of Gram-negative bacilli from turbid urine samples using VITEK2. J Lab Physicians [serial online] 2018 [cited 2019 Oct 16];10:299-303. Available from: http://www.jlponline.org/text.asp?2018/10/3/299/236099
| Introduction|| |
Urinary tract infections (UTIs) are among the most common infectious diseases occurring in either the community or healthcare setting. Urine culture needs up to 18–24 h of incubation, and if further testing is required, pathogen identification (ID) and antimicrobial susceptibility testing (AST) need an additional 24–48 h. Consequently, the initial antibiotic therapy is mostly empirical.
Based on the knowledge of the local spectrum of pathogens and the prevalence of antimicrobial resistance, UTIs are commonly treated empirically. Due to increasing antibiotic resistance to commonly used oral agents, empiric treatment has no guaranteed effect on the pathogen.,,
With inappropriate antibiotic prescriptions, healthcare cost increases and quality of care decreases. To avoid the use of broad-spectrum antibiotics and to optimize antibiotic treatment, microbiology laboratory should develop procedures with short turnaround time for AST.
For this reason, microbiologists have for many years tried to reduce the turnaround time of bacterial ID and susceptibility testing. At the beginning, microscopic, chemical, and new automated methods provided rapid and cost-effective alternatives to standard culture techniques for detecting bacteriuria.,, Gram staining of concentrated urine samples has demonstrated high sensitivity with high positive predictive value for the quick and cheap diagnosis of UTIs. Recently, a real-time PCR method and matrix-assisted laser desorption/ionization time-of-flight mass-spectrometry (MALDI-TOF MS) have been described for the direct ID of bacterial species from urine samples.,, However, additional time is required for antibiotic susceptibility testing.
We studied a rapid method using the VITEK 2 system (Biomérieux), which has been widely introduced in most clinical microbiology laboratories, for ID and susceptibility testing of urinary tract pathogens directly from urine specimens of patients with suspected UTI selected by pyuria and screened by Gram staining and compared with the conventional method.
| Materials and Methods|| |
The study was conducted at Nizam's Institute of Medical Sciences from October 2016 to February 2017. A total of 4594 urine samples received to the microbiology laboratory were screened, out of which, 864 were turbid and 62 samples met the inclusion criteria.
Turbid urine samples with turbidity >0.5 McFarland, Gram stain showing pus cells, Gram-negative (GN) bacilli of single morphotype.
Absence of pus cells and organisms, > one morphotype of GN Bacilli, candiduria, and turbidity <0.5 McFarland.
The turbid urine specimen fitting into the criteria was taken in a sterile test tube. Ten microliter was centrifuged in a sterile tube at 10,000 g for 10 min. Supernatants were discarded. Ten microliter of the centrifuged urine specimen was spotted on a glass slide with a 0.01-mL calibrated loop. Smears were air dried, heat fixed, Gram stained and examined under oil immersion for bacterial counts. The samples were directly inoculated onto VITEK 2 cards for ID and AST. The same sample were incoculated onto CHROMagar and incubated at 37°C for 24 h. The next day, pure growth from the culture plate was inoculated onto VITEK 2 cards for ID and AST, and both the results were compared.
The bacterial pellet was collected with a cotton swab to prepare a 0.5 McFarland suspension in 3.5 mL of 0.45% saline using the Densichek plus (Biomerieux).
At this point, the VITEK 2 cards were inoculated following the instructions of the manufacturer. Cards were selected depending on Gram stain analysis. When GN rods were observed, IDGN were used. AST-N280 (antimicrobial susceptibility testing) was the card employed for antibiotic susceptibility testing of GN bacilli.
Standard inoculation method
About 10-μL volume of a urine sample was spread onto solid chromogenic agar media (CPS3; Biomérieux). The inoculated plates were incubated at 37°C for 24 h. In cases of monomicrobial growth after incubation, a standardized 0.5 McFarland inoculum was prepared from the agar medium, and the VITEK 2 cards mentioned above were inoculated following the manufacturer's recommendations. The microbiological culture was considered as the reference method for ID and antibiotic susceptibility testing.
Periodically, all cards studied were tested with ATCC Escherichia More Details coli 25922 and ATCC Pseudomonas aeruginosa 27853.
Bacterial ID and susceptibility data from the direct method were compared with those obtained from the standard test.
The ID results with the direct method were grouped into three different categories:
- Complete agreement: The strain ID with the direct VITEK 2 test and with the reference method was consistent at genus and species level
- Misidentification: The strain ID with both systems was discrepant at the genus and the species level
- Not identified: No ID was given at all.
Susceptibility results for all isolates tested were characterized into three clinical categories (susceptible [S], intermediate [I], or resistant [R]) according to the criteria of the VITEK 2 advanced expert system.
Discrepancies were classified as follows:
- Very major errors (VM): They were defined as an S result with the direct VITEK 2 test and an R result according to the reference method;
- Major error (ME): They were defined as an R result with the direct VITEK 2 test and an S result according to the reference method.
- Minor error (MinE): Any other category discrepancy was defined as a minor error (MinE).
Agreements were classified as follows:
- Essential agreement (EA): Minimal Inhibitory Concentration (MIC) obtained for the direct method was within ±1 2-fold dilution of the conventional method 
- Categorical agreement (CA): MIC from direct method in the same susceptibility category as the conventional method 
- Precision EA (PEA): Agreement within +/-1 2-fold dilution of the precision test isolate antibiotic MIC
- Precision CA (PCA): Agreement with the interpretative results of the precision test isolate using Clinical Laboratory Standard Institute (CLSI) criteria.
Based on the US Food and Drug Administration (FDA)/CLSI approved criteria, the overall ME ≤5% is acceptable, EA and CA ≥90%, PEA and PCA ≥95%, ME and MinE <10%.
To assess the precision of ID and susceptibility five test samples with three ATCC strains of E. coli, Klebsiella pneumoniae, and P. aeruginosa were run in triplicate daily for 5 days.
The following antimicrobial agents were compared: Amoxicillin–clavulanic acid, ampicillin, piperacillin-tazobactam, cefuroxime, cefuroxime axetil, ceftriaxone, cefoperazone-sulbactam, cefepime, ciprofloxacin, ertapenem, imipenem, meropenem, gentamicin, amikacin, nalidixic acid, nitrofurantoin, and trimethoprim-sulfamethoxazole [Table 1].
|Table 1: Concentration ranges of the used antimicrobial agents (antimicrobial susceptibility testing N280)|
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| Results|| |
A total of 844 turbid urines received to microbiology laboratory were screened, out of which, 62 samples met the inclusion criteria. All the samples which met the inclusion criteria were processed by both routine and direct methods. Among these 62 samples, polymicrobial growth was observed in 3 samples, and two samples were sterile, so these 5 samples were excluded, and finally, 57 samples were analyzed. E. coli was the predominant isolate (41, 71.92%), followed by K. pneumoniae (10, 17.54%). These microorganisms of the Enterobacteriaceae family could be accurately identified directly from urine within 4.5 h.
The overall complete agreement for ID was 80.7% [Table 2]. Of these 57 isolates, 7 were misidentified (12.2%) and 4 were unidentified (7%) by direct method. Out of the 7 misidentified isolates, 5 were E. coli and 2 were K. pneumoniae. E. coli was misidentified as Rauotella, Sphingomonas, Yersinia More Details, and Serratia, and K. pneumoniae was misidentified as K. oxytoca.
|Table 2: Identification of Gram-negative isolates by routine and direct method|
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The isolates which showed complete agreement for ID were analyzed for AST agreement. About 46 isolates showed complete agreement and these isolates were evaluated for susceptibility EA, complete agreement (Categorical agreement (CA); sensitive, intermediate, resistant), VM error, major error (ME), minor error (MinE), and precision.
For the 46 g negative isolates AST, EA was 97.3% and CA 94.3%. EA was 100% for the 7 antimicrobial drugs, 97.8% for 4 drugs. CA was 100% for the 3 antimicrobial drugs [Table 3].
|Table 3: Antimicrobial susceptibility testing agreement between direct and standard for Gram-negative bacteria using VITEK N280|
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VM errors were reported with cefepime, ertapenem, and imipenem. All were from the same isolate K. pneumoniae. Major errors were commonly reported for piperacillin-tazobactam followed by ertapenem, imipenem, meropenem, and cotrimoxazole. These major errors were reported in E. coli, K. pneumoniae, and P. aeruginosa. Minor errors were reported commonly for piperacillin-tazobactam and nitrofurantoin followed by amoxyclav. Combined error rate was 5.62%.
PEA and PCA were 99% for all drugs combined.
| Discussion|| |
This study was done to assess the reliability of the VITEK 2 system for direct ID and antibiotic susceptibility testing of urinary tract pathogens (directly) from the urine sediment. E. coli was the predominant isolate (71.92%) which was comparable (56.8%) to the study conducted by Niranjan and Malini.
The results of this study showed that the microorganisms of the Enterobacteriaceae family, most often responsible for the urinary infection, can be accurately identified directly from urine within 4.5 h using the VITEK 2 direct system, with 80.7% correlation with the standard technique. Compared with other ID systems directly from urine specimens, the 80.7% correlation rate for Enterobacteriaceae is similar or slightly lower than that obtained by others.,,,
Overall, 7 microorganisms were misidentified or not identified by the direct method while it was 19 in the study conducted by Munoz-Dávila et al.
Cellular elements, inhibitory substances, or pH changes in urine could have contributed to the false-positive and false-negative reactions responsible for misidentification.
In addition, the criteria stated by Jorgensen and Ferraro and those from the CLSI and FDA recommend that a new test method should provide 90% EA with MIC values determined by the reference method. The susceptibility CA was 94.3% and EA was 97.3% rate found in this study which was similar to Munoz-Dávila et al.
VM errors, major error, and minor errors of 0.5%, 2.2%, 3%, respectively, were reported in this study. Munoz-Dávila et al. reported 0.2% VM errors and 0.4% major errors.
The combined ME and MinE rate was 5.6% while it was 1.04% in the study conducted by Bazzi et al.
In summary, the results of this study indicate that the organisms can be accurately identified directly from an inoculum prepared from centrifuged urine specimens. Susceptibility test data may also be obtained that compare favorably with routine VITEK 2 susceptibility results performed on isolated colonies of the same organism. This method permits the reporting of the complete results on urine specimens with GN bacilli as early as 8 h after their arrival in the laboratory. The most important benefit is the faster pathogen ID and susceptibility data, which allow earlier selective antimicrobial therapy. The impact of rapid procedures on patient care, laboratory efficiency, and cost containment is significant.
This direct method could be very useful in those microbiology laboratories with a high number of urine specimens from the emergency department (highly), which employ the VITEK 2 system as the reference method for bacterial ID and antibiotic susceptibility testing. This direct test might be an excellent alternative to the automated urine screening systems and to the MALDI-TOF MS technology, both techniques require special hardware therefore high investment costs for most laboratories.
However, before inoculating the VITEK 2 card, several data had to be collected (microscopy of the primary urine, selecting for monomorphology, and bacterial count).
Limitations of the study
The study was conducted with a smaller sample size, because of this low number, further studies are necessary to assess the reliability of the VITEK 2 system for direct ID of other GN, nonfermentative rods and Gram-positive cocci.
| Conclusion|| |
With a 94.3% agreement for AST for common urinary tract pathogens and a reduced turnaround time by 24 h, the direct inoculation of the VITEK 2 system had a potential clinical benefit for initiating timely and appropriate antibiotic therapy for UTI. This could benefit both patient and hospital in providing better treatment and reducing the cost.
Financial support and sponsorship
Conflicts of interest
There are no conflicts of interest.
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[Table 1], [Table 2], [Table 3]