|Year : 2019 | Volume
| Issue : 2 | Page : 138-143
Detection of VIM and NDM-1 metallo-beta-lactamase genes in carbapenem-resistant Pseudomonas aeruginosa clinical strains in Bahrain
Ronni Mol Joji1, Nouf Al-Rashed1, Nermin Kamal Saeed2, Khalid Mubarak Bindayna1
1 Department of Microbiology, Immunology and Infectious Disease, College of Medicine and Medical Sciences, Arabian Gulf University, Manama, Kingdom of Bahrain
2 Department of Pathology, Salmaniya Medical Complex, Ministry of Health, Manama, Kingdom of Bahrain
|Date of Submission||13-Sep-2018|
|Date of Acceptance||03-Jan-2019|
|Date of Web Publication||27-May-2019|
Dr. Ronni Mol Joji
Department of Microbiology, Immunology and Infectious Disease, College of Medicine and Medical Sciences, Arabian Gulf University, Manama
Kingdom of Bahrain
Source of Support: None, Conflict of Interest: None
| Abstract|| |
INTRODUCTION: Carbapenem-resistant Pseudomonas aeruginosa has emerged as a life-threatening infectious agent worldwide. Carbapenemase genes are reported to be some of the most common mechanisms for carbapenem resistance in P. aeruginosa. No reports are available from the Kingdom of Bahrain about carbapenem resistance and the underlying cause. In this study, we determined to study the presence of the metallo-beta-lactamase (M b L) genes of VIM family and NDM-1 in carbapenem-resistant P. aeruginosa strains.
METHODOLOGY: Fifty carbapenem-resistant P. aeruginosa isolates were obtained from three main hospitals of Bahrain. They were subjected to antimicrobial susceptibility testing by disc diffusion test. Subsequently, MβL was detected by imipenem-ethylene diamine tetraacetic acid (EDTA) combined disc test and conventional polymerase chain reaction.
RESULTS: Among 50 P. aeruginosa strains, 40 (80%) were imipenem resistant. Among the 40 imipenem-resistant strains, 35 (87.5%) strains were positive for the imipenem-EDTA combined disc test, and 21 (52%) were carrying MβL genes. Nineteen (47.5%) strains were positive for the VIM gene; one (2.5%) strain was carrying the NDM-1 gene, while one strain was carrying both the VIM and NDM-1 genes. None of the imipenem sensitive strains carried the VIM or NDM-1 gene.
CONCLUSION: This is the first study to report the presence of the VIM family gene and NDM-1 genes in imipenem-resistant P. aeruginosa isolates in the Kingdom of Bahrain. The study also confirms the multiple drug resistance by the MβL strains, attention should therefore from now on, be focused on prevention of further spread of such isolates by firm infection control measures, and to reduce its threat to public health.
Keywords: Carbapenem resistance, NDM-1 gene, Pseudomonas aeruginosa, VIM gene
|How to cite this article:|
Joji RM, Al-Rashed N, Saeed NK, Bindayna KM. Detection of VIM and NDM-1 metallo-beta-lactamase genes in carbapenem-resistant Pseudomonas aeruginosa clinical strains in Bahrain. J Lab Physicians 2019;11:138-43
|How to cite this URL:|
Joji RM, Al-Rashed N, Saeed NK, Bindayna KM. Detection of VIM and NDM-1 metallo-beta-lactamase genes in carbapenem-resistant Pseudomonas aeruginosa clinical strains in Bahrain. J Lab Physicians [serial online] 2019 [cited 2019 Oct 19];11:138-43. Available from: http://www.jlponline.org/text.asp?2019/11/2/138/258946
| Introduction|| |
Carbapenem resistance among Pseudomonas aeruginosa is a global health threat and has led to therapeutic limitations. Carbapenemase genes which code for carbapenemases is reported to be an important mechanism in carbapenem resistance of P. aeruginosa.
Carbapenemases are assigned to three classes of β lactamases: Ambler A, B, and D. Class B metallo-beta-lactamases (MβL) include the enzymes that belong to VIM, IMP, SPM, GIM, and NDM families. They hydrolyze all β-lactams, except aztreonam, and this activity can be inhibited by ethylene diamine tetraacetic acid (EDTA). Nothing is known about P. aeruginosa MβL producers in the Kingdom of Bahrain.
The most relevant epidemiologically and clinically important MβL types are VIM (Verona integrin-encoded MβL), IMP (imipenemase), NDM (New Delhi MβL), and SPM (Sao Paulo MβL).,
Here, we studied the VIM family and NDM-1 genes among MβL-producing P. aeruginosa isolates by phenotypic test and polymerase chain reaction (PCR).
| Methodology|| |
The study was conducted following ethical approval from the Ethical Review Board of Arabian Gulf University (E014-PI-11/16). The study was conducted on 50 nonduplicate P. aeruginosa strains isolated from clinical samples from 50 patients (included community, wards, and ICU patients) attending the Salmaniya Medical Complex, King Hamad University Hospital, and Bahrain Defense Force Hospital, located in the Kingdom of Bahrain. The isolates were preserved in 20% skimmed milk with glycerol solution and stored in a freezer at −80°C until further processing.
This study included all the P. aeruginosa strains isolated from patients of all the age groups and both the sexes. This included both the outpatients and the inpatients attending all the three hospitals in Bahrain.
Repeat isolates from the same patients were excluded from the study.
The samples were collected under complete aseptic conditions and included wound swabs, sputum, deep tracheal aspirates, endotracheal tube, urine, blood, and tissue.
Identification of Pseudomonas aeruginosa
The isolates were identified as P. aeruginosa strains by standard laboratory techniques such as Gram staining, colony morphology, cetrimide test, catalase test, oxidase reaction, citrate utilization, TSI reaction, oxidation-fermentation test, gelatin hydrolysis test, polymyxin B sensitivity testing, and sugar fermentation tests.
Antimicrobial susceptibility testing
Antimicrobial susceptibility testing was performed by the disc diffusion method on Mueller-Hinton agar (MHA) plates and interpreted according to Clinical Laboratory Standards Institute recommendations (CLSI 2016). The antibiotic discs used were as follows: imipenem (10 μg), meropenem (10 μg), amikacin (30 μg), gentamycin (10 μg), ceftazidime (30 μg), cefotaxime (30 μg), ciprofloxacin (5 μg), norfloxacin (10 μg), piperacillin + tazobactum (100/10 μg), tigecycline (15 μg), and colistin (10 μg).
Phenotypic detection of metallo-beta-lactamase activity
All the isolates resistant to imipenem (zone size ≤15 mm as per the CLSI guidelines 2016) by disc diffusion method on MHA were screened for MβL activity by imipenem-EDTA combined disc test (IMP-EDTA CDT) as described by Yong et al. In brief, an overnight culture of the test organism was compared with 0.5 McFarland which is comparable to the density of bacterial suspension 1.5 × 108 CFU/ml and was inoculated on an MHA plate. Two imipenem discs (10 μg) were placed on the inoculated plate at a distance of 5 cm from each other, and 10 μl of 0.5M EDTA solution was added to one of the discs. The plates were incubated at 35°C for 16–18 h. The inhibition zones of each disc were compared, and the test isolates which showed a zone size of ≥7 mm for IMI-EDTA disc as compared to imipenem disc alone were considered as MβL positive [Figure 1]. P. aeruginosa ATCC 27853 strain was used as the control strain.
|Figure 1: Photo of a Combined Disc Test showing strain A as metallo-beta-lactamase producer and strain B as non metallo-beta-lactamase producer|
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Polymerase chain reaction assay for detection of metallo-beta-lactamase genes of VIM family and NDM-1
Conventional PCR testing of all the isolates for the presence of the VIM family and NDM-1 genes was done as per manufacturer's instructions. The sequence of primers specific for VIM family and NDM-1 used in this study is listed in [Table 1]. Total DNA of all the bacterial isolates was extracted by the boiling method. The extracted DNA was then stored at −20°C until further processing. Amplification was done using GoTaq Green PCR Master Mix (Promega). The PCR mix consisted of 25 μl master mix, 1 μl each of forward and reverse primer, 5 μl template DNA and nuclease-free water to make a final volume of 50 μl. The thermal cycler program was as follows: initial denaturation at 95°C for 5 min, 35 cycles of denaturation at 95°C for 30 s, annealing at 53°C for 30 s, and extension at 72°C for 30 s, followed by final extension at 72°C for 10 min. Agarose gel electrophoresis for the detection of amplicons was done by separating 10 μl of each amplicon and 100 bp ladder on a 1.5% agarose gel. Amplicons were visualized using ultraviolet transilluminator and subsequently analyzed.
|Table 1: Polymerase chain reaction primers for amplification of VIM and NDM-1 genes|
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Data were analyzed using the Statistical Package for the Social Science (SPSS) version 20 (IBM, Armonk, NY, United States of America) to obtain descriptive data. Kappa test was used to measure the level of agreement between the phenotypic and genotypic test.
| Results|| |
The majority of the Pseudomonas aeruginosa strains are imipenem resistant
Fifty nonduplicate P. aeruginosa strains were isolated from clinical sources. The antimicrobial susceptibility test of these samples shows that out of 50 P. aeruginosa strains, 40 (80%) were imipenem resistant. All the isolates were resistant to ciprofloxacin (100%). 90% of the strains were resistant to norfloxacin, meropenem, and piperacillin/tazobactam which is shown in [Table 2].
|Table 2: Antibiotic resistance pattern of fifty isolates to various antibiotics|
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Most of the imipenem-resistant strains are positive for metallo-beta-lactamase
To determine whether imipenem resistance is caused by the production of MβL or by other mechanisms, the 40 imipenem-resistant strains were analyzed with the imipenem-EDTA combined disc test. An example of the imipenem-EDTA combined disc test is shown in [Figure 1]. This phenotyping revealed that 35 (88%) of these 40 strains were positive for MβL.
More than half of the imipenem resistance is due to VIM family and NDM-1 genes
Genotyping of all 50 strains was performed to determine the presence of the VIM family and NDM-1 genes. In [Figure 2] and [Figure 3], the analyses of the PCR products are illustrated for the VIM and NDM-1 genes, respectively. The results showed that of the 40 imipenem-resistant strains, 19 (47.5%) were positive for the VIM gene, one isolate (2.5%) for the NDM-1 gene, and one (2.5%) was carrying both the VIM and NDM-1 genes, as shown in [Table 3]. None of the imipenem sensitive strains were carrying these genes.
|Figure 2: Polymerase chain reaction products after agarose gel electrophoresis. Lanes 1-5 and 7 show one band with molecular size 390 bp (VIM gene), lane 6 is a VIM negative strain, and lane 8 is water (negative control). Lane L contains a 100 bp ladder|
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|Figure 3: Polymerase chain reaction products after agarose gel electrophoresis. Lanes 2 and 6 show one band with molecular size 445 bp (NDM-1 gene, lanes 1,3,4,5, and 7 are NDM-1 negative strains and lane 8 is water (negative control). Lane L contains a 100 bp ladder|
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|Table 3: Distribution of VIM and NDM-1 genes in 50 Pseudomonas aeruginosa strains by using polymerase chain reaction|
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Many of the VIM and NDM-1 gene-positive MβL producers were isolates obtained from endotracheal aspirate (seven of 19 VIM-positive strains [37%] and one of two NDM-1-positive strains [50%]) as can be seen in [Table 4]. These MβL producers were also resistant to most of the other antibiotics tested while they were all sensitive to colistin [Table 5].
|Table 5: Antibiotic susceptibility pattern of isolated metallo-beta-lactamase producing Pseudomonas aeruginosa strains|
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Correlation of the phenotypic test results with polymerase chain reaction
When comparing the phenotypic test results with genotypic test results, we found that the strength of agreement was fair between the two tests (kappa value = 0.47) as shown in [Table 6]. The sensitivity and specificity of the combined disc test in relation to PCR was 100% and 51.72%, respectively.
|Table 6: Comparison of the results of the combined disc test with polymerase chain reaction|
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| Discussion|| |
P. aeruginosa is a multidrug-resistant organism causing nosocomial infections. Over the past decades, the resistance to carbapenems is increasing and has become a major health threat. Early detection of MβL producers is therefore very crucial for optimal treatment of infections, and this will further reduce the resistance rate and prevent nosocomial spread. In the present study, out of 50 P. aeruginosa isolates, 40 (80%) were found to be resistant to imipenem which is in agreement with the study by Polotto et al. in Brazil where they reported 96.4% of their strains as imipenem resistant. Another study, in India, by Arunagiri et al. reported 62.7% of the isolates as resistant to imipenem. In Egypt, EL-Mosallamy et al. conducted a study on 100 strains, wherein they found 25 (25%) strains imipenem resistant. In Saudi Arabia, Mohamed et al. reported that imipenem resistance rate was 38.6% in 2011, while 5 years later, another study reported that 91% of 33 P. aeruginosa isolates were resistant to imipenem.
There are no official standard guidelines for MβL detection. PCR analysis is the gold standard, but it is not practiced in routine microbiology laboratories. We therefore first used IMI-EDTA CDT phenotyping for MβL screening and compared the results with the genotyping results. By IMI-EDTA CDT phenotyping, we observed that out of 40 imipenem-resistant strains, 35 (88%) produced MβL whereas Pitout et al. from Canada found that 110/241 (46%) imipenem-resistant strains were MβL positive while in Iran, Saderi et al. reported that 65/100 (65%) of their imipenem-resistant strains were MβL positive using phenotypic methods. Another study by Panchal et al. compared different phenotypic tests for MβL detection and found that 19/30 (63.33%) were positive by IMI-EDTA combined disc test.
The PCR results revealed that out of 40 imipenem-resistant strains, 19 (47.5%) strains were positive for the VIM gene which is similar to a study from Egypt by Essa and Afif where they found 40% of their imipenem-resistant strains carrying the VIM gene. Al-Agamy et al. from Saudi Arabia reported 20.6% of the imipenem-resistant strains as MβL producers and all the MβL strains were found to carry the VIM gene. Furthermore, in a study by Tawfik et al. from Saudi Arabia, VIM was found in all the 15 MβL-positive isolates (100%). In a study in Canada, 43% of the strains were positive for the VIM gene.
Resistance transferred by the NDM-1 gene is also a growing public health problem. The main reservoir is the Indian subcontinent, and the secondary reservoirs are the Balkans regions and the Middle East. Here, we observed only one isolate (2.5%) positive for NDM-1 gene which is in corroboration with a study from Egypt by Zafer et al. which concluded that the prevalence of the NDM-1 gene was only 4.2%. Another study by Shanthi et al. from India in 2014 reported that only four isolates out of 61 were positive for NDM-1. We observed only one isolate that carried both the VIM and NDM-1 genes, whereas in Saudi Arabia, Shaaban et al. reported 8 out of 16 imipenem-resistant strains carrying both NDM-1 and VIM subtypes (VIM 1 and VIM 2). A few previous studies have also reported the presence of multiple carbapenemase genes in P. aeruginosa, including the KPC and VIM in Colombia and SPM-1, KPC-2, and VIM-2 in Brazil. The differences in the incidence and the types of genes seen in MβL producing strains are likely due to the geographical variations and differences in antibiotic usage.
The strength of agreement between the combined disc test and PCR is moderate. The sensitivity and specificity of IMI-EDTA CDT in relation to PCR is 100% and 51.72%, respectively, which was similar to the studies conducted by Pandya et al. and Arunagiri et al. where they reported sensitivity of IMI-EDTA CDT as 96.3% and 94%, respectively, while Picão et al. reported a lower sensitivity of 80%.
| Conclusion|| |
This is the first study to report the presence of VIM and NDM-1 in imipenem-resistant P. aeruginosa strains in the kingdom of Bahrain. The test results also showed that imipenem-EDTA combined disc test is a sensitive method for the detection of MβL producers. This test can, therefore, be used as an alternative to PCR in diagnostic laboratories. The study also identified the multiple drug resistance of the MβL producers. Attention should be focused on early detection of MβL producers to prevent further spread of such multidrug-resistant strains. The development of strong antimicrobial stewardship programs is essential, with emphasis on the importance of infection control measures to prevent further spread of these strains.
Financial support and sponsorship
This study was supported by Arabian Gulf University.
Conflicts of interest
There are no conflicts of interest.
| References|| |
Kateete DP, Nakanjako R, Namugenyi J, Erume J, Joloba ML, Najjuka CF, et al.
Carbapenem resistant Pseudomonas aeruginosa
and Acinetobacter baumannii
at mulago hospital in Kampala, Uganda (2007-2009). Springerplus 2016;5:1308.
Rizek C, Fu L, Dos Santos LC, Leite G, Ramos J, Rossi F, et al.
Characterization of carbapenem-resistant Pseudomonas aeruginosa
clinical isolates, carrying multiple genes coding for this antibiotic resistance. Ann Clin Microbiol Antimicrob 2014;13:43.
Manenzhe RI, Zar HJ, Nicol MP, Kaba M. The spread of carbapenemase-producing bacteria in Africa: A systematic review. J Antimicrob Chemother 2015;70:23-40.
Qu TT, Zhang JL, Wang J, Tao J, Yu YS, Chen YG, et al.
Evaluation of phenotypic tests for detection of metallo-beta-lactamase-producing Pseudomonas aeruginosa
strains in china. J Clin Microbiol 2009;47:1136-42.
Walsh TR, Toleman MA, Poirel L, Nordmann P. Metallo-beta-lactamases: The quiet before the storm? Clin Microbiol Rev 2005;18:306-25.
Elsherif R, Ismail D, Elawady S, Jastaniah S, Al-Masaudi S, Harakeh S,et al
. Boronic acid disk diffusion for the phenotypic detection of polymerase chain reaction-confirmed, carbapenem-resistant, gram-negative bacilli isolates. BMC Microbiol 2016;16:135.
Yong D, Lee K, Yum JH, Shin HB, Rossolini GM, Chong Y, et al.
Imipenem-EDTA disk method for differentiation of metallo-beta-lactamase-producing clinical isolates of Pseudomonas
spp. And Acinetobacter
spp. J Clin Microbiol 2002;40:3798-801.
EL-Mosallamy W, Osman A, Tabl H, AL-Tabbakh A. Phenotypic and genotypic methods for detection of metallo-beta-lactamase (MBL) producing Pseudomonas aeruginosa
. Egypt J Med Microbiol 2015;24:27-35.
Ahmed OB, Dablool AS. Quality improvement of the DNA extracted by boiling method in gram negative bacteria. Int J Bioassays 2017;6:5347-9.
Hammami S, Boutiba-Ben Boubaker I, Ghozzi R, Saidani M, Amine S, Ben Redjeb S, et al.
Nosocomial outbreak of imipenem-resistant Pseudomonas aeruginosa
producing VIM-2 metallo-β-lactamase in a kidney transplantation unit. Diagn Pathol 2011;6:106.
Lister PD, Wolter DJ, Hanson ND. Antibacterial-resistant Pseudomonas aeruginosa
: Clinical impact and complex regulation of chromosomally encoded resistance mechanisms. Clin Microbiol Rev 2009;22:582-610.
Polotto M, Casella T, de Lucca Oliveira MG, Rúbio FG, Nogueira ML, de Almeida MT, et al.
Detection of P. Aeruginosa harboring bla CTX-M-2, bla GES-1 and bla GES-5, bla IMP-1 and bla SPM-1 causing infections in Brazilian tertiary-care hospital. BMC Infect Dis 2012;12:176.
Arunagiri K, Sekar B, Sangeetha G, John J. Detection and characterization of metallo-beta-lactamases in Pseudomonas aeruginosa
by phenotypic and molecular methods from clinical samples in a tertiary care hospital. West Indian Med J 2012;61:778-83.
Mohamed AA, Shibl AM, Zaki SA, Tawfik AF. Antimicrobial resistance pattern and prevalence of metallo-β-lactamases in Pseudomonas aeruginosa
from Saudi Arabia. Afr J Microbiol Res 2011;5:5528-33.
Al-Agamy MH, Jeannot K, El-Mahdy TS, Samaha HA, Shibl AM, Plésiat P, et al.
Diversity of molecular mechanisms conferring carbapenem resistance to Pseudomonas aeruginosa
isolates from Saudi Arabia. Can J Infect Dis Med Microbiol 2016;2016:4379686.
Pitout JD, Gregson DB, Poirel L, McClure JA, Le P, Church DL, et al.
Detection of Pseudomonas aeruginosa
producing metallo-beta-lactamases in a large centralized laboratory. J Clin Microbiol 2005;43:3129-35.
Saderi H, Lotfalipour H, Owlia P, Salimi H. Detection of Metallo-β-Lactamase Producing Pseudomonas aeruginosa
Isolated From Burn Patients in Tehran, Iran. Lab Med 2010;41:609-12.
Panchal CA, Oza SS, Mehta SJ. Comparison of four phenotypic methods for detection of metallo-β-lactamase-producing gram-negative bacteria in rural teaching hospital. J Lab Physicians 2017;9:81-3.
] [Full text]
Al-Agamy MH, Shibl AM, Zaki SA, Tawfik AF. Antimicrobial resistance pattern and prevalence of MBL in P. aeruginosa
from Saudi Arabia. Afr J Microbiol Res 2011;30:5528-33.
Tawfik AF, Shibl AM, Aljohi MA, Altammami MA, Al-Agamy MH. Distribution of ambler class A, B and D β-lactamases among Pseudomonas aeruginosa
isolates. Burns 2012;38:855-60.
Dortet L, Poirel L, Nordmann P. Worldwide dissemination of the NDM-type carbapenemases in gram-negative bacteria. Biomed Res Int 2014;2014:249856.
Zafer MM, Al-Agamy MH, El-Mahallawy HA, Amin MA, Ashour MS. Antimicrobial resistance pattern and their beta-lactamase encoding genes among Pseudomonas aeruginosa
strains isolated from cancer patients. Biomed Res Int 2014;2014:101635.
Shanthi M, Sekar U, Kamalanathan A, Sekar B. Detection of new delhi metallo beta lactamase-1 (NDM-1) carbapenemase in Pseudomonas aeruginosa
in a single centre in southern india. Indian J Med Res 2014;140:546-50.
] [Full text]
Shaaban M, Al-Qahtani A, Al-Ahdal M, Barwa R. Molecular characterization of resistance mechanisms in Pseudomonas aeruginosa
isolates resistant to carbapenems. J Infect Dev Ctries 2017;11:935-43.
Correa A, Montealegre MC, Mojica MF, Maya JJ, Rojas LJ, De La Cadena EP, et al.
First report of a Pseudomonas aeruginosa
isolate coharboring KPC and VIM carbapenemases. Antimicrob Agents Chemother 2012;56:5422-3.
Pandya N, Prajapati S, Mehta S, Kikani K, Joshi P. Evaluation of various methods for detection of (MβL) production in gram negative bacilli. Egypt J Med Microbiol 2011;2:775-7.
Picão RC, Andrade SS, Nicoletti AG, Campana EH, Moraes GC, Mendes RE, et al.
Metallo-beta-lactamase detection: Comparative evaluation of double-disk synergy versus combined disk tests for IMP-, GIM-, SIM-, SPM-, or VIM-producing isolates. J Clin Microbiol 2008;46:2028-37.
[Figure 1], [Figure 2], [Figure 3]
[Table 1], [Table 2], [Table 3], [Table 4], [Table 5], [Table 6]