Year : 2018 | Volume
: 10 | Issue : 4 | Page : 370--373
HBG2 -158 (C>T) polymorphism and its contribution to fetal hemoglobin variability in Iraqi Kurds with beta-thalassemia minor
Dilan J Albarawi1, Amer A Balatay2, Nasir Al-Allawi3
1 Scientific Research Center, College of Science, University of Duhok, Duhok, Iraq
2 Department of Pathology, College of Pharmacy, University of Duhok, Duhok, Iraq
3 Department of Pathology and Scientific Research Center, College of Medicine, University of Duhok, Duhok, Iraq
PURPOSE: Hemoglobin (Hb) F% is increased in up to half of beta-thalassemia (β-thal) carriers. Several polymorphisms have been linked to such variability in different populations, including HBG2 - 158(C>T) (Xmn I polymorphism) on chromosome 11. To determine the role of this polymorphism in such variability among Iraqi Kurds, the current study was initiated.
MATERIALS AND METHODS: A total of 102 consecutive patients diagnosed as β-thal minor were enrolled. The enrollees had their diagnosis based on peripheral blood counts and high-performance liquid chromatography to determine HbA2 and HbF. All enrollees had their DNA extracted by phenol-chloroform method and Xmn I polymorphism detected by restriction fragment length polymorphism-polymerase chain reaction.
RESULTS: The mean age (standard deviation [SD]) of the 102 enrollees was 25.4 (14.0) years, and the enrollees included 48 males and 54 females. Xmn I polymorphism was identified in heterozygous state in 46 (45.1%) patients and in homozygous state in one patient (0.98%). Thus, the minor allele frequency of this polymorphism was 0.235 in the studied group. There were no significant differences in red cell indices and HbA2% in carriers of the minor allele compared to noncarriers, while HbF% and absolute HbF concentrations were significantly higher in the former subgroup (P = 0.032 and 0.014, respectively). This polymorphism's contribution to HbF variability was found to be 5.8% in the studied sample. Furthermore, those with HbF ≥2% were 3.2 folds more likely to carry the minor allele.
CONCLUSIONS: Xmn I polymorphism is frequently encountered in Iraqi Kurds with β-thal minor, and it is significantly associated with higher fetal hemoglobin in these patients.
Prof. Nasir Al-Allawi
Department of Pathology, College of Medicine, University of Duhok, 9 Azadi Hospital Road, 1014 AM, Duhok
|How to cite this article:|
Albarawi DJ, Balatay AA, Al-Allawi N. HBG2 -158 (C>T) polymorphism and its contribution to fetal hemoglobin variability in Iraqi Kurds with beta-thalassemia minor.J Lab Physicians 2018;10:370-373
|How to cite this URL:|
Albarawi DJ, Balatay AA, Al-Allawi N. HBG2 -158 (C>T) polymorphism and its contribution to fetal hemoglobin variability in Iraqi Kurds with beta-thalassemia minor. J Lab Physicians [serial online] 2018 [cited 2019 Mar 23 ];10:370-373
Available from: http://www.jlponline.org/article.asp?issn=0974-2727;year=2018;volume=10;issue=4;spage=370;epage=373;aulast=Albarawi;type=0