Journal of Laboratory Physicians

ORIGINAL ARTICLE
Year
: 2018  |  Volume : 10  |  Issue : 4  |  Page : 387--391

Prevalence of blaKPC and its occurrence with other beta-lactamases in Klebsiella pneumoniae


Poothakuzhiyil Remya, Mariappan Shanthi, Uma Sekar 
 Department of Microbiology, Sri Ramachandra Medical College and Research Institute, Chennai, Tamil Nadu, India

Correspondence Address:
Mrs. Poothakuzhiyil Remya
Department of Microbiology, Sri Ramachandra Medical College and Research Institute, Chennai - 600 116, Tamil Nadu
India

BACKGROUND: Klebsiella pneumoniae (K. pneumoniae) is an important nosocomial pathogen, and the emergence of multidrug resistance in these organisms limits the treatment options for serious infections caused by them. K. pneumoniae carbapenemase (KPC) is one of the clinically significant Class A beta-lactamases. AIM AND OBJECTIVE: This study was aimed to detect the KPC and its coexistence with other beta-lactamases in K. pneumoniae. MATERIALS AND METHODS: A total of 370 isolates, collected over a period of 1 year, were included in this study. The source of these isolates were urine (n = 170), exudative specimens (n = 132), respiratory secretions such as bronchial wash, endotracheal aspirate, and pleural fluid (n = 38), and blood (n = 30). For all the isolates, antibiotic susceptibility tests by disc diffusion, modified Hodge test, and KPC screening test were done. Polymerase chain reaction (PCR) was performed for the detection of KPC and the copresence of other beta-lactamases genes. RESULTS: Among the 370 isolates, 41 were resistant to the carbapenem by disc diffusion and minimum inhibitory concentration tests. Screen test using ertapenem and the boronic acid disk was positive in 14 isolates. Only one isolate harbored KPC gene by PCR, and it was co-produced with SHV-12 and CTX-M-15. CONCLUSION: PCR remains the gold standard for detection of KPC compared with any other phenotypic methods. Early detection of these genes helps in initiating proper antibiotic treatment.


How to cite this article:
Remya P, Shanthi M, Sekar U. Prevalence of blaKPC and its occurrence with other beta-lactamases in Klebsiella pneumoniae.J Lab Physicians 2018;10:387-391


How to cite this URL:
Remya P, Shanthi M, Sekar U. Prevalence of blaKPC and its occurrence with other beta-lactamases in Klebsiella pneumoniae. J Lab Physicians [serial online] 2018 [cited 2018 Dec 13 ];10:387-391
Available from: http://www.jlponline.org/article.asp?issn=0974-2727;year=2018;volume=10;issue=4;spage=387;epage=391;aulast=Remya;type=0